Page Views: 139 Date Added: 07/22/2011 Last Updated: 07/25/2011 Expiry Date: 07/19/2021 Product/Service Details Aralia chinensis Extract Active Ingredient: Oleanolic acid Oleanol Specification: 98% Test Method UV-VIS oleanolic acid Herb Source Olea europaea L. Swertia mileensis T.N. Ho et W. L. Shi Ligustrum lucidum Ait oleanolic acid Synonyms Oleanol Caryophyllin oleanolic acid Molecular Formula and Molecular Weight C30H48O3£»456.71 oleanolic acid Melting Point 308¡ãC-310¡ãC oleanolic acid Solubility Insoluble in water£¬Sol in ether£¬acetone£¬chloroform and ethanol oleanolic acid Brief Structural Class£ºTriterpene Appearance£ºWhite crystal oleanolic acid Pharmacology Protect liver and depress enzyme£¬ Anti-inflammation, Diuretic,Anti-cancer Two triterpene acids, oleanolic acid (OA) and ursolic acid (UA), purified components from several Botanical drugs with known antitumor activity such as, were examined for their ability and possible pathway on inhibiting the tumor growth of hepG2 cells. Cells were treated with OA and UA with doses of 100 and 200 ug/ml for 12, 24 and 36 hrs, respectively. The changes of DNA fragmentation, apoptotic bodies, DNA synthesis and the expression of cell cycle related genes were investigated after various treatments. Decrease of DNA synthesis in cells treated with both OA and UA was obviously observed at these doses for 24 and 36 hrs by using BrdU staining method. Total RNA were purified with RNA Zol B reagent and the first stranded cDNA were synthesized by AMV transcriptase. Semiquantitation of the cDNA was performed by primer dropping method. Both mRNA and protein of the CDK inhibitor of INK4 family which specifically inhibits CDK4/6 activity and then reduces the phosphorylation of RB, p-15INK4b, in hepG2 cells were greatly induced by OA and UA. However, the expression of another member in this family, p-16INK4a, was not significantly induced. Other CDK inhibitors such as p-21CIP, p-27KIP and cell cycle related genes were not induced after various OA and UA treatments. The DNA fragmentation and apoptotic bodies were also found in this study with both the time- and dose-dependent phenomena. These results suggested that the apoptosis induced by OA and UA might be partially mediated through PKC pathway and associated with p-15INK4b gene induction. Ursolic acid (UA) and oleanolic acid (OA), isolated from Glechoma hederacea, inhibited Epstein-Barr virus activation induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) in mouse skin. The inhibitory effects were evaluated for 20 weeks. Continuous application of UA and OA (41 nmol of each) before TPA-treatment (4.1 nmol) delayed the formation of papillomas in mouse skin and reduced the rate (%) of papilloma bearing mice. Both UA and OA exhibited remarkable inhibitory activity against tumor promotion, which is comparable to the known tumor inhibitor, retinoic acid (RA). Compared to either RA or OA, ursolic acid inhibited tumors more effectively after a single application before initial TPA-treatment. This suggests that the role of tumor inhibition by UA differs from that of either RA or OA. It is suggested that pretreatment of skin with UA may inhibit the first dramatic cellular event in tumor promotion caused by TPA1. Contact:Sallycheng CRGANIC HERB INC www.organic-herb.com Tel:86-731-82739210 Fax:86-731-82739211 Mobile:86-15111035952(working 24 hours ) MSN:sallycheng0426@hotmail.com Skype:sallycheng104 Mail:sallycheng@organic-herb.com

Company Information (Organic Herb Inc)

Company Profile: Globe Supplier for Plant Extracts,Herbal & Botanical Extracts,Organic Herb,Mushroon Extract,Animal Extract from China-Organic Herb Inc. Organic Herb Inc. (OHI) has been dedicated to the research and development of innovative nutraceutical ingredients since 1993. Our commitment to science and quality control has earned OHI recognition within the industry as a trusted supplier of ingredients of the highest quality. OHI is the Developer, Manufacturer, Exporter and Primary Supplier of the most remarkable alternative and complementary herbal health products and raw material available today. OHI develops and manufactures superior nutraceutical ingredients. OHI has advanced in-home lab as well as professional R&D people swift response to any of your request. We also co-operates with the world's most prestigious universities and private research institutions in the biological assessment of safety and effectiveness up to clinical phase II. We offer science-based ingredients, unconditional customer guarantee as well as excellent technical support, most of our products are tested by USA independent labs. We have very good relationship with famous USA independent Labs, and we are also member of NNFA (USA) as well as APHA. We have our own FDA registered Number. OHI’s major standardizedGreen Tea Extract, EGCG, Ginkgo Biloba Extract ,Luo Han Guo Extract 80% Mogrosides 25% Mogrosides, Black Sesame Extract 90% Sesamin, Flaxseed Extract 25% Lignans SDG Secoisolariciresinol Diglycoside, Honeysuckle Flower Extract 99% Chlorogenic acid, 5-HTP, Resveratrol 99%, Ginseng Root Extract 80% Ginsenosides, Grape Seed Extract 95% OPC, Soy Bean Extract 40% Isoflavones, Huperzine A 99%, Tanshinone II A 80%, Synephrine 90%, Puerarin 97%, Rhodiola Rosea Extract, Pueratiae Root Extract, Echinacea Extract, Bitter Melon Extract, Citrus Aurantium Extract, Cordyceps Extract, Epimedium Extract, Red Clover Extract, Apple Bark Extract 40% phloretin, Galanthamine, Banaba Extract 1% Corosolic Acid, Loquat Leaf Extract 25% ursolic acid, Bilberry Extract 25% Anthocyanidins, Black Tea Extract 40% Theaflavines, Garlic Extract (freeze Dried), Macleaya cordata Extract, Piper Nigrum Extract 90% piperine, Lycopene, Yohimbine, Artemisinin, Shikimic acid,Eurycoma longifolia Extract and so on ..... are well sale more than 30 countries throughout the world.. >> More Website URL: http://www.bridgat.com/u41138